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Updated: May 13, 2026
Remote Laboratory Management: Respiratory Virus Diagnostics
Published on: April 6, 2019
[Establishment of a high-throughput respiratory virus detection technology without RNA purification and reverse
Dan-li Yang1, Xiao-yi Tian, Wei-xian Shi
1Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Beijing, China.
Objective:
To establish a convenient and high-throughput respiratory virus detection method to facilitate epidemiological viral monitoring.
Methods:
We used high-throughput microsphere-based flexible multi-analyte profiling technology (xMAP) coupled with signal amplification molecules to simultaneously detect RNAs of 8 viruses including influenza viruses A and B, parainfluenza viruses type 1, 2 and 3, respiratory syncytial viruses A and B, and metapneumovirus in a 96-well plate format. The sensitivity and specificity of the method for the synthetic viral RNAs were evaluated.
Results:
There was no cross-reactivity among the 8 respiratory viral target RNAs. The detection limits for the 8 viral in intro-transcribed RNAs ranged from 1204 to 4695 RNA copies.
Conclusion:
We establish a sensitive, specific, convenient, and high-throughput multiplex detection method suitable for detecting multiple respiratory viral RNAs for epidemiological viral monitoring.
