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Related Experiment Video

Updated: May 13, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Hierarchical molecular tagging to resolve long continuous sequences by massively parallel sequencing.

Sverker Lundin¹, Joel Gruselius, Björn Nystedt

¹Science for Life Laboratory, KTH, Gene Technology, Solna, Sweden.

Scientific Reports

|March 9, 2013

Summary

This study introduces hierarchical molecular tagging to extend DNA read lengths on short-read sequencing platforms. This method enables sequencing continuous DNA regions over 3000 bp, improving variant detection and genomic analysis.

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Last Updated: May 13, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

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Published on: March 15, 2019

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Short-read sequencing technologies have limitations in determining long, continuous DNA sequences.
Accurate variant calling and genome assembly are hindered by short read lengths.

Purpose of the Study:

To develop a novel method for extending read lengths using short-read sequencing.
To enable the analysis of longer contiguous DNA regions than conventionally possible.

Main Methods:

Hierarchical molecular tagging of DNA libraries.
Controlled degradation and timed sub-sampling of indexed libraries.
Nested indexing to record sample, molecular, and degradation information.

Main Results:

Demonstrated the ability to decode continuous DNA regions exceeding 3000 bp using Illumina sequencing.
Successfully applied the method to variant calling in lambda phage genomes.
Analyzed TP53 variants in cancer cell lines and targeted canine mitochondrial DNA.

Conclusions:

Hierarchical molecular tagging is an effective strategy for overcoming short-read length limitations.
This protocol significantly enhances the utility of massive sequencing systems for complex genomic analyses.
The method has broad applicability in various fields, including cancer research and population genetics.