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Published on: July 13, 2010
Establishing an in vivo p48ZnF bioluminescence mouse brain imaging model
1Department of Biomedical Engineering, College of Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea. klaus@hanyang.ac.kr
Abstract:
p48ZnF is a C3H1 zinc finger domain-containing protein that is involved in the control of gene transcription and translation. In the present study a novel transgenic p48ZnF mouse model is described that is useful for in vivo brain imaging using luciferase as bioluminescence-mediating reporter gene. Yeast two-hybrid screening and western blot analyses revealed Drg1 (developmentally regulated GTP binding protein 1) and Pcbp1 (poly (rC)-binding protein 1) as p48ZnF-associated proteins. Interestingly, p48ZnF' cellular location of action depends on the cell's differentiation status: nuclear in proliferating cells and cytoplasmic in differentiated neurons.
