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Updated: May 13, 2026
Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Published on: March 23, 2015
Cytotoxic effects of secosterols and their derivatives on several cultured cells
Susumu Tomono1, Yukari Yasue, Noriyuki Miyoshi
1Laboratory of Biochemistry, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, Graduate Program in Food and Nutritional Sciences, University of Shizuoka, Japan.
Abstract:
The cytotoxic effects of various oxysterols on several culture cells were examined. Ozonolysis products of cholesterol, secosterols (3β-hydroxy-5-oxo-5,6-secocholestan-6-al) and its aldolization product (3β-hydroxy-5β-hydroxy-B-norcholestane-6β-carboxaldehyde) and their keto alcohol and acid derivatives, were found to have potent cytotoxic activities, as compared with major endogenous oxysterols such as 5β,6β-epoxycholesterol, 7β-hydroxycholesterol, 7-ketocholesterol, and 25-hydroxycholesterol. Secosterols might play important roles in tissue damage and inflammation-associated diseases.
