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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Analysis of ascorbic acid biosynthesis using a simple transient gene expression system in tomato fruit protoplasts
Shingo Sakamoto1, Yukichi Fujikawa, Muneharu Esaka
1Graduate School of Biosphere Sciences, Hiroshima University, Higashi-hiroshima, Japan.
Bioscience, Biotechnology, and Biochemistry
|March 9, 2013
Abstract:
We established a new method of transient expression using tomato fruit protoplasts. The method showed that L-ascorbic acid (AsA) content in tomato protoplasts was increased by transient expression of the L-galactose-1-phosphate phosphatase gene. This system provides a means of rapid analysis to clarify the function of AsA biosynthetic enzymes and AsA roles in tomato fruit.
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