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Updated: May 13, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
Published on: June 27, 2014
Improved analysis of excited state proton transfer kinetics by the combination of standard and convolution methods
Adilson A Freitas1, António A L Maçanita, Frank H Quina
1Centro de Química Estrutural, Instituto Superior Técnico, Universidade Técnica de Lisboa, 1049-001 Lisbon, Portugal. adilsondefreitas@ist.utl.pt
Abstract:
We wish to report a powerful methodology for analysis and interpretation of complex fluorescence decays and other kinetic data, based on the addition of convolution analysis to standard (individual and global) analysis. The method was applied to cases involving excited state proton transfer of flavylium salts in water, in water-1,4-dioxane mixtures and in micellar solutions of sodium dodecyl sulfate (SDS). The results clearly support the proposal of the intermediacy of a proton-base geminate pair in environments other than water.
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