Related Experiment Video
Updated: May 13, 2026

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
A simultaneous analysis method for Caihu-Dayuan-Yin using reversed-phase high-performance liquid chromatography
Ha-Jeong Kwon1, Ji-Hwan Kim, Yong-Duk Park
1Department of Preventive and Social Dentistry, Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
Abstract:
A simultaneous analysis method was developed for Caihu-Dayuan-Yin decoction containing Bupleuri Radix, Glycyrrhizae Radix, and Ponciri fructus by reversed-phase (RP) HPLC coupled with pulsed amperometric detection (PAD). The analytes were separated in a RP column with 0.01 % phosphoric acid-acetonitrile gradient elution. The limits of detection (S/N = 3) and of quantification (S/N = 10) ranged 0.1-3.0, and 0.3-10 ng, respectively. The intra- and interday precisions (RSDs) were ≤15.15 % and the average recoveries were 94.6-98.3 %. Our RP-HPLC-PAD method showed 1.7-100 times higher sensitivity than the RP-HPLC-UV method.
Related Concept Videos
High-Performance Liquid Chromatography: Types of Detectors
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation
Gas Chromatography: Types of Detectors-I
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Ion-Exchange Chromatography
High-Performance Liquid Chromatography: Elution Process
