Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Postmortem changes in brain catecholamine enzymes.

I B Black, S C Geen

    Archives of Neurology
    |January 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Postmortem brain enzyme activity varies by type. Tyrosine hydroxylase and dopa decarboxylase decline rapidly, while dopamine-beta-hydroxylase remains stable for extended periods after death.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Substance P levels differ in sympathetic target organ terminals and ganglion perikarya.

    Brain research·2013
    Same author

    Regulation of synaptic molecular architecture in a rat sympathetic ganglion and hippocampus.

    Journal of cognitive neuroscience·2013
    Same author

    Visual experience specifically regulates synaptic molecules in rat visual cortex.

    Journal of cognitive neuroscience·2013
    Same author

    Dissociation of motor hyperactivity and spatial memory deficits by selective hippocampal lesions in the neonatal rat.

    Journal of cognitive neuroscience·2013
    Same author

    Fate of amnion-derived stem cells transplanted to the fetal rat brain: migration, survival and differentiation.

    Journal of cellular and molecular medicine·2008
    Same author

    Long-term cryopreserved amniocytes retain proliferative capacity and differentiate to ectodermal and mesodermal derivatives in vitro.

    Molecular reproduction and development·2006
    Same journal

    Incorrect Table Entries and Word.

    Archives of neurology·2016
    Same journal

    IDEAL for CCSVI Research-Reply.

    Archives of neurology·2013
    Same journal

    Atlas of Inherited Metabolic Diseases, 3rd ed.

    Archives of neurology·2013
    Same journal

    Error in byline: in heterogeneity of coenzyme q10 deficiency: patient study and literature review.

    Archives of neurology·2013
    Same journal

    This month in archives of neurology.

    Archives of neurology·2013
    Same journal

    About this journal.

    Archives of neurology·2013
    See all related articles

    Area of Science:

    • Neuroscience
    • Biochemistry

    Background:

    • Enzyme activity changes after death are crucial for interpreting postmortem brain studies.
    • Understanding the stability of key enzymes in neurotransmitter synthesis is essential.

    Purpose of the Study:

    • To investigate the postmortem stability of tyrosine hydroxylase (TH), dopa decarboxylase (DDC), and dopamine-beta-hydroxylase (DBH) in specific rat brain regions.
    • To establish the time course of activity decay for these enzymes following death.

    Main Methods:

    • Assessed enzyme activities in rat brain samples at various postmortem intervals.
    • Focused on brain areas rich in catecholaminergic neurons: caudate-putamen, substantia nigra, and locus ceruleus.

    Main Results:

    • Tyrosine hydroxylase activity showed an exponential decrease with a half-life of 2-4 hours.

    Related Experiment Videos

  • Dopa decarboxylase activity remained stable for 5 hours before a rapid decline.
  • Dopamine-beta-hydroxylase activity was notably stable, remaining within 20% of control for at least 20 hours postmortem.

    • Conclusions:

    • The differential postmortem stability of TH, DDC, and DBH has significant implications for research methodologies.
    • DBH exhibits remarkable stability, making it a reliable marker in postmortem brain tissue for extended periods.