Related Experiment Video
Updated: May 13, 2026

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
Published on: September 2, 2016
Measurement of pore diffusivity in separation media for high performance liquid chromatography
Kanji Miyabe1, Yuko Matsumoto, Nobuho Ando
1Department of Chemistry, Faculty of Science, Rikkyo University, Tokyo, Japan. kmiyabe@rikkyo.ac.jp
Abstract:
It was demonstrated that the peak parking-moment analysis (PP-MA) method could be used as an alternative for the measurement of pore diffusivity (D(p)) in porous packing materials for HPLC. The value of D(p) of uracil was measured by the PP-MA method under non-retained conditions in the RPLC systems using C18-silica gel particles and C18-silica monolith as the stationary phase and 70 vol% methanol as the mobile phase. The value of D(p) was compared with that derived by the pulse response-moment analysis (PR-MA) method under the same conditions. The D(p) values of uracil derived by the PP-MA and PR-MA methods were almost in agreement with each other. Because the PP-MA and PR-MA methods have complementary characteristics as experimental methods for studying mass transfer kinetics, it is expected that the combination of the two methods would lead to an effective strategy for the measurement of D(p) in porous separation media for HPLC.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Analyte Adsorption and Distribution
Diffusion on Chromatography Columns
Longitudinal diffusion occurs when the solute molecules in the mobile phase diffuse from the more concentrated center of the chromatographic band to the more dilute regions on either side, both towards and against the flow direction. This...
High-Performance Liquid Chromatography: Elution Process
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
High-Performance Liquid Chromatography: Instrumentation

