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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: May 13, 2026

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
08:25

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

Efficient and specific gene knockdown by small interfering RNAs produced in bacteria.

Linfeng Huang1, Jingmin Jin, Padraig Deighan

  • 1Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts, USA.

Nature Biotechnology
|March 12, 2013
PubMed
Summary

Researchers developed a novel bacterial production method for potent small interfering RNAs (siRNAs). This method yields highly effective siRNAs for gene silencing applications, offering a new avenue for research and potential therapeutics.

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Last Updated: May 13, 2026

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
08:25

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Bacterial Delivery of RNAi Effectors: Transkingdom RNAi
07:56

Bacterial Delivery of RNAi Effectors: Transkingdom RNAi

Published on: August 18, 2010

Area of Science:

  • Molecular Biology
  • Biotechnology
  • RNA Interference

Background:

  • Synthetic small interfering RNAs (siRNAs) are crucial for studying gene function and developing gene-silencing therapies.
  • Current siRNA production relies primarily on chemical synthesis.
  • There is a need for alternative, scalable methods for producing functional siRNAs.

Purpose of the Study:

  • To establish a method for producing highly potent siRNAs in Escherichia coli.
  • To evaluate the efficacy and safety of bacterially produced siRNAs in mammalian cells.
  • To explore the potential of this method for targeting polymorphic genes.

Main Methods:

  • Ectopic expression of the siRNA-binding protein p19 in E. coli.
  • Stabilization of bacterial RNase III-generated siRNA species by p19.
  • Co-expression of a hairpin RNA encoding the target gene sequence.
  • Transfection of mammalian cells with bacterially produced siRNAs.

Main Results:

  • Bacterially produced siRNAs effectively silenced target gene expression by approximately 90%.
  • The produced siRNAs demonstrated no immunogenicity or off-target effects.
  • The method successfully generated siRNAs targeting a hairpin RNA of over 200 nucleotides.
  • Bacterial production yielded siRNAs with multiple sequences against a target gene.

Conclusions:

  • Bacterial production of siRNAs using p19 is a viable and potent method.
  • This approach offers a scalable and potentially cost-effective alternative to chemical synthesis.
  • Bacterially produced siRNAs hold promise for therapeutic applications, particularly for polymorphic genes.