Related Experiment Video
Updated: May 13, 2026
Experimental Methods for Testing the Effects of Neurotrophic Peptide, ADNF-9, Against Alcohol-induced Apoptosis during Pregnancy in C57BL/6 Mice
Published on: April 24, 2013
The protective effects of grape seed extract on MDA, AOPP, apoptosis and eNOS expression in testicular torsion: an
Firuze Bayatli1, Derya Akkuş, Eser Kilic
1Assisted Reproductive Technology Center, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
Objectives:
Grape seed proanthocyanidin extract (GSPE) is a potent antioxidant and a free radical scavenger. This study was designed to determine whether GSPE could protect against dysfunction and oxidative stress induced by torsion-detorsion injury in rat testis.
Methods:
A total of 45 male Wistar albino rats were divided into five groups: control group, sham group, torsion-detorsion (T/D) group, T/D + GSPE group, GSPE group. GSPE was administrated 100 mg/kg/day with oral gavage over seven days before torsion. Testicular torsion was performed for 2 h, and afterward, detorsion was performed for 2 h. The rats were decapitated under ketamine anesthesia, and their testes tissues were removed. Tissue malondialdehyde, advanced oxidation protein products levels, eNOS expression, apoptosis and histopathological damage scores were then compared.
Results:
Testicular torsion-detorsion caused significant increases in malondialdehyde level, apoptosis and eNOS expression level and caused a significant decrease in advanced oxidation protein product levels and testicular spermatogenesis in ipsilateral testes. GSPE prevented the rise in malondialdehyde, apoptosis and eNOS expression and improved testicular morphology and Johnsen's score.
Conclusions:
As a result, testicular torsion gives rise to serious damage in testes and GSPE is a potent antioxidant agent in preventing testicular injury.
Insights
Grape seed proanthocyanidin extract (GSPE) protects against testicular torsion-detorsion injury. GSPE reduced oxidative stress, apoptosis, and improved testicular morphology and function.
Area of Science:
- Urology
- Oxidative Stress Research
- Pharmacology
Background:
- Testicular torsion-detorsion injury causes significant oxidative stress and damage.
- Grape seed proanthocyanidin extract (GSPE) is known for its antioxidant and free radical scavenging properties.
Purpose of the Study:
- To investigate the protective effects of GSPE against testicular dysfunction and oxidative stress induced by torsion-detorsion injury in a rat model.
- To evaluate GSPE's impact on key markers of oxidative stress, apoptosis, and testicular histology.
Main Methods:
- Male Wistar albino rats were divided into five groups: control, sham, torsion-detorsion (T/D), T/D + GSPE, and GSPE.
- GSPE (100 mg/kg/day) was administered orally for seven days prior to inducing torsion for 2 hours followed by 2 hours of detorsion.
- Tissue levels of malondialdehyde, advanced oxidation protein products, eNOS expression, apoptosis, and histopathological damage were assessed.
Main Results:
- Torsion-detorsion significantly increased malondialdehyde, apoptosis, and eNOS expression while decreasing advanced oxidation protein products and spermatogenesis.
- GSPE administration effectively prevented the increase in malondialdehyde, apoptosis, and eNOS expression.
- GSPE treatment led to improved testicular morphology and a higher Johnsen's score.
Conclusions:
- Testicular torsion-detorsion injury results in severe testicular damage.
- GSPE demonstrates potent antioxidant capabilities and is effective in preventing testicular injury induced by torsion-detorsion.