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High-throughput fluorescence assay of cytochrome P450 3A4
Qian Cheng1, F Peter Guengerich
1Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 12, 2013
Abstract:
Microtiter plate-based fluorescence assays allow rapid measurement of the catalytic activities of cytochrome P450 oxygenases (P450s). We describe a high-throughput fluorescence assay of P450 3A4, one of the key enzymes involved in xenobiotic metabolism. The assay involves the oxidative debenzylation of 7-hydroxy-4-trifluoromethyl coumarin, producing an increase in fluorescence.
