Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evaluating pulmonary vascular permeability with radiolabeled proteins: an error analysis.

M A Mintun1, T E Warfel, D P Schuster

  • 1Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, Missouri 63110.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|April 1, 1990
PubMed
Summary

Mathematical simulation reveals positron emission tomography (PET) is superior for measuring pulmonary transcapillary escape rate (PTCER). The slope-intercept (SI) method introduces significant errors due to ignoring protein back-flux.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Florbetapir F 18 amyloid PET and 36-month cognitive decline: a prospective multicenter study.

Molecular psychiatry·2014
Same author

Functional neuroanatomy of verbal free recall: A replication study.

Human brain mapping·2014
Same author

Early metabolic improvement following bariatric surgery in morbidly obese adolescents.

Pediatric blood & cancer·2011
Same author

Effect of bariatric surgery on normal and abnormal renal function.

Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery·2011
Same author

Improving CSF biomarker accuracy in predicting prevalent and incident Alzheimer disease.

Neurology·2011
Same author

Alzheimer disease identification using amyloid imaging and reserve variables: proof of concept.

Neurology·2010

Area of Science:

  • Physiology
  • Medical Imaging
  • Mathematical Modeling

Background:

  • Pulmonary vascular permeability is crucial for lung function.
  • Accurate measurement of transvascular protein flux is essential for assessing lung health.
  • Existing methods for calculating pulmonary transcapillary escape rate (PTCER) have limitations.

Purpose of the Study:

  • To compare the accuracy of two methods for evaluating pulmonary vascular permeability: Positron Emission Tomography (PET) and the slope-intercept (SI) method.
  • To identify sources of error in PTCER calculations for both methods.
  • To determine the optimal method for assessing pulmonary vascular permeability.

Main Methods:

  • Mathematical simulation was employed to compare PET and SI methods.

Related Experiment Videos

  • Both methods utilize intravenously administered radiolabeled proteins and external radiation detection.
  • PET data were interpreted using a two-compartment model, while SI method used linear regression.

    • Main Results:

    • The SI method can produce significant errors in PTCER calculation by neglecting protein back-flux.
    • Both methods are prone to errors if vascular volumes not involved in tracer exchange are included in analysis.
    • The PET method can also yield errors if pulmonary vascular volume changes significantly during data acquisition, especially with near-normal permeability.

    Conclusions:

    • The PET method is the preferred choice for accurately measuring pulmonary vascular permeability via protein flux.
    • Despite potential errors, both PET and SI methods may hold clinical utility.
    • Further refinement of data analysis is needed to minimize errors in both techniques.