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Treatment of antibodies to reduce non-specific binding in immunoassays using the avidin-biotin complex

D M Bodmer1, L X Tiefenauer

  • 1Medical Bioanalytics Project, Paul Scherrer Institute, Villigen, Switzerland.

Journal of Immunoassay
|January 1, 1990
PubMed

Three different methods to reduce non-specific binding of antibodies to avidin coated plates in an immunoassay are described and compared: The use of a blocking agent, antiserum adsorption on avidin-agarose and antiserum adsorption to avidin by a so called pre-incubation method. The pre-incubation method proved to be highly efficient, fast and simple. Further applications of this method to different immunoassay systems are proposed in the discussion section.

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