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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 13, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Infrared-based protein detection arrays for quantitative proteomics.

Ulrike Korf1, Christian Löbke, Florian Haller

  • 1German Cancer Research Center (DKFZ), Department of Molecular Genome Analysis/B050, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany +49 6221 424765 ; +49 6221 423454 ; u.korf@dkfz.de.

Expert Opinion on Drug Discovery
|March 14, 2013
PubMed
Summary
This summary is machine-generated.

Protein microarrays offer sensitive, specific quantification for proteomics. This review highlights advancements in reverse phase protein arrays and their applications in tumor biology and drug discovery.

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Last Updated: May 13, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Area of Science:

  • Proteomics
  • Biotechnology
  • Molecular Biology

Background:

  • Protein microarrays are emerging as a standard method in quantitative proteomics.
  • This antibody-based platform enables sensitive and specific quantification of target proteins.
  • High sample capacity and parallel array production minimize sample consumption.

Purpose of the Study:

  • To summarize recent progress in reverse phase protein arrays (RPPA).
  • To focus on normalization measures in infrared-based RPPA with quantitative readout.

Main Methods:

  • Review of advancements in reverse phase protein array technology.
  • Focus on normalization strategies for quantitative readout.

Main Results:

  • RPPA technology has advanced significantly.
  • Normalization measures are crucial for accurate quantitative readout.

Conclusions:

  • Protein microarrays are poised to benefit tumor biology and drug discovery.
  • Comprehensive pathway analysis using protein microarrays will be facilitated.