Related Experiment Video
Updated: May 13, 2026
Identifying Protein-protein Interaction Sites Using Peptide Arrays
Published on: November 18, 2014
Infrared-based protein detection arrays for quantitative proteomics
Ulrike Korf1, Christian Löbke, Florian Haller
1German Cancer Research Center (DKFZ), Department of Molecular Genome Analysis/B050, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany +49 6221 424765 ; +49 6221 423454 ; u.korf@dkfz.de.
Background:
Protein microarrays have the potential to join the field of quantitative proteomics as a standard method. This antibody-based experimental platform allows the highly sensitive and highly specific quantification of selected target proteins and excels with high sample capacity. As a key feature, numerous arrays can be produced in parallel, thus minimizing sample consumption.
Objective:
The recent progress made in the field of reverse phase protein arrays is summarized, with a focus on the introduction of normalization measures, as introduced in the authors' infrared-based protein arrays with quantitative readout approach.
Conclusion:
Tumor biology as well as drug discovery applications will soon benefit from the comprehensive description of signaling pathways and protein microarrays are an appropriate tool to achieve this goal.
