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Updated: May 13, 2026

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Real-time RT-PCR threshold cycles value for Kir6.1 from the blood correlates with parameters of vascular function: a
Faisel Khan1, Wen Ling Choong, Qingyou Du
1Medical Research Institute, Division of Cardiovascular and Diabetic Medicine, Ninewells Hospital & Medical School, University of Dundee, Dundee DD1 9SY, UK.
Abstract:
Abstract We examined the presence of KATP channel subunits, Kir6.1 and SUR2B, mRNAs in the blood and vascular function in healthy volunteers (41 males, 34 females). Real-time reverse transcriptase (RT)-PCR threshold cycles (Ct) was used as an indicator of mRNA levels. Baseline skin perfusion and the post-occlusion reactive hyperemia response exhibited a significant positive correlation with Ct for Kir6.1. There was no correlation between Kir6.1 Ct and brachial artery flow-mediated dilatation. Gender had no influence on relationships between blood Kir6.1 Ct and vascular function. We conclude that blood Kir6.1 mRNA levels could be potentially used as a biomarker of the vascular function.
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