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Updated: May 13, 2026

AQRNA-seq for Quantifying Small RNAs
Published on: February 2, 2024
RNA-Seq: revelation of the messengers
Marcel C Van Verk1, Richard Hickman, Corné M J Pieterse
1Plant-Microbe Interactions, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Abstract:
Next-generation RNA-sequencing (RNA-Seq) is rapidly outcompeting microarrays as the technology of choice for whole-transcriptome studies. However, the bioinformatics skills required for RNA-Seq data analysis often pose a significant hurdle for many biologists. Here, we put forward the concepts and considerations that are critical for RNA-Seq data analysis and provide a generic tutorial with example data that outlines the whole pipeline from next-generation sequencing output to quantification of differential gene expression.
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