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Related Experiment Video

Updated: May 13, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

Promoter reuse in prokaryotes.

Harm Nijveen¹, Mariana Matus-Garcia, Mark Willem John van Passel

¹Laboratory of Bioinformatics; Wageningen University; Wageningen, The Netherlands.

Mobile Genetic Elements

|March 14, 2013

Summary

Scientists found over 4000 promoter groups in prokaryotic genomes. Around 6% are shared across diverse bacteria, suggesting mobile genetic elements facilitate transcriptional regulation rewiring.

Area of Science:

Genomics
Molecular Biology
Evolutionary Biology

Background:

Gene transcriptional regulation is crucial for cellular function.
Promoters, DNA sequences regulating gene expression, are typically associated with specific genes.
Anecdotal evidence suggests promoter reuse independent of their original genes, hinting at flexible regulatory mechanisms.

Purpose of the Study:

To identify and categorize conserved promoter groups across prokaryotic genomes.
To investigate the extent of promoter sharing across different bacterial taxa.
To explore the potential role of mobile genetic elements in promoter mobility and gene regulation.

Main Methods:

Conducted a conservative sequence similarity search across all fully sequenced prokaryotic genomes.

Keywords:

horizontal gene transfer mobile elements prokaryotes promoters rewiring

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Last Updated: May 13, 2026

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Identified over 4000 groups of highly similar promoters.

Performed database searches against known mobile elements and RNA motifs (e.g., riboswitches).

Main Results:

Over 4000 highly similar promoter groups were identified.
Approximately 6% of these promoter groups are shared between bacteria from different taxonomic depths (genera to phyla).
Evidence suggests that regulatory motifs, like riboswitches, can be associated with mobile promoters.

Conclusions:

A significant number of promoter groups are conserved and shared across diverse bacterial lineages.
The mobility of promoters, potentially facilitated by mobile genetic elements, offers a mechanism for rapid gene regulatory rewiring.
This finding has implications for understanding bacterial adaptation and evolution through flexible transcriptional control.