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Related Experiment Video

Updated: May 13, 2026

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Published on: May 21, 2018

Heme proteins in lactic acid bacteria.

Michael Baureder¹, Lars Hederstedt

¹Microbiology Group, Department of Biology, Lund University, Lund, Sweden.

Advances in Microbial Physiology

|March 14, 2013

Summary

Lactic acid bacteria (LAB) can utilize heme for protein function but do not synthesize it for growth. This unique heme physiology in LAB offers potential applications in food and medicine.

Area of Science:

Microbiology
Biochemistry

Background:

Lactic acid bacteria (LAB) are crucial in food production and medicine.
Unlike many bacteria, LAB do not synthesize heme (protoheme IX) or depend on it for growth.
LAB can incorporate exogenous heme into specific heme-containing proteins.

Purpose of the Study:

To elucidate the cellular functions of identified heme proteins in LAB.
To discuss the biogenesis of these heme proteins.
To explore applications stemming from LAB's unique heme metabolism.

Main Methods:

Analysis of heme protein identification in LAB.
Investigation of heme incorporation pathways.
Functional characterization of heme proteins.

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Published on: October 23, 2016

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Last Updated: May 13, 2026

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Published on: May 21, 2018

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

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Main Results:

LAB possess a limited set of heme proteins.
Heme incorporation is dependent on exogenous heme supply.
Identified heme proteins contribute to specific cellular functions within LAB.

Conclusions:

LAB exhibit an extraordinary heme physiology distinct from other bacteria.
This unique characteristic presents novel opportunities for biotechnological and medical applications.
Understanding LAB heme metabolism is key to harnessing its potential.