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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Single shot high resolution digital holography.

Kedar Khare1, P T Samsheer Ali, Joby Joseph

  • 1Department of Physics, Indian Institute of Technology Delhi, New Delhi 110016, India. kedark@physics.iitd.ac.in

Optics Express
|March 14, 2013
PubMed
Summary
This summary is machine-generated.

A new computational method recovers high-resolution images from single digital holograms. This technique excels even with overlapping frequency terms, offering clear images with minimal noise.

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Last Updated: May 13, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

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Area of Science:

  • Computational imaging
  • Digital holography
  • Image processing

Background:

  • Digital holography records 3D information optically.
  • Traditional hologram reconstruction can suffer from artifacts like DC and twin images.
  • Image quality is often limited by overlapping frequency terms in the hologram.

Purpose of the Study:

  • To develop a novel computational method for high-resolution image recovery from single digital hologram frames.
  • To overcome limitations of physical hologram replay, particularly with overlapping spectral terms.
  • To achieve high-quality image reconstruction with reduced artifacts and noise.

Main Methods:

  • Solving a constrained optimization problem to obtain the complex object field.
  • Utilizing a computational approach distinct from physical hologram replay.
  • Applying the method to a Fresnel zone hologram recorded with a small off-axis angle.

Main Results:

  • Successful high-resolution image recovery from a single hologram frame.
  • Effective separation of object field even when DC and cross terms overlap in the Fourier domain.
  • Excellent image recovery observed without DC or twin image terms.
  • Minimal speckle noise in the reconstructed images.

Conclusions:

  • The proposed computational method enables high-quality, artifact-free image recovery from digital holograms.
  • This approach offers a significant advancement over traditional physical reconstruction techniques.
  • The method is robust even under challenging recording conditions, such as overlapping spectral components.