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Updated: May 13, 2026

Biochemical Measurement of Neonatal Hypoxia
Published on: August 24, 2011
[Measurement of acetonitrile in blood and urine by head-space gas chromatography]
Ru-Xin Luo1, Xian-Yi Zhuo, Bao-Hua Shen
1School of Pharmacy, Medical College of Soochow University, Suzhou 215123, China. luoruxin1986@sina.cn
Objective:
To establish the method for measurement of acetonitrile in blood and urine by head-space gas chromatography.
Methods:
DB-ALC1 (30 m x 320 microm x 1.8 microm) and DB-ALC2 (30 m x 320 microm x 1.2 microm) capillary column were used to measure the acetonitrile in blood and urine with the isopropanol as internal standard reference.
Results:
The limits of detection of acetonitrile in both blood and urine were 0.5 microg/mL, with a linear range of 5-1000 microg/mL (r = 0.999).The accuracy of this method was 93.2%-98.0%. The RSD for the intra-day and inter-day were less than 3.7%.
Conclusion:
The method is capable for measurement analysis of acetonitrile in blood and urine.
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