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Published on: July 3, 2015
Brain hsp70 expression and DNA damage in mice exposed to extremely low frequency magnetic fields: a dose-response
Milena Villarini1, Maria Vittoria Ambrosini, Massimo Moretti
1Department of Medical-Surgical Specialties and Public Health, Perugia, Italy.
Purpose:
To determine whether a dose-response relationship exists among exposure to extremely low frequency magnetic fields (ELF-MF) at different densities and 70-kDa heat shock protein (hsp70) expression and DNA damage in mouse brain.
Materials And Methods:
Male CD1 mice were exposed to ELF-MF (50 Hz; 0.1, 0.2, 1 or 2 mT) for 7 days (15 h/day) and sacrificed either at the end of exposure or after 24 h. Hsp70 expression was determined in cerebral cortex-striatum, hippocampus and cerebellum by real-time reverse-transcriptase polymerase chain reaction (RT-PCR) and Western blot analysis. Primary DNA damage was evaluated in the same tissues by comet assay. Sham-exposed mice were used as controls.
Results:
No changes in both hsp70 mRNA and corresponding protein occurred following exposure to ELF-MF, except for a weak increase in the mRNA in hippocampus of exposed mice to 0.1 mT ELF-MF. Only mice exposed to 1 or 2 mT and sacrificed immediately after exposure presented DNA strand breaks higher than controls in all the cerebral areas; such DNA breakage reverted to baseline in the mice sacrificed 24 h after exposure.
Conclusions:
These data show that high density ELF-MF only induce reversible brain DNA damage while they do not affect hsp70 expression.
