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Published on: August 10, 2018
[Verification of multipotential mesenchymal stem cell presence in peripheral blood of rabbits]
Xiao-Hui Liu1, Tao Zhang, Ning Fang
1Zunyi Medical College Affiliated Hospital, Zunyi, Guizhou Province, China.
Abstract:
The aim of this study was to verify the presence of multipotential mesenchymal stem cells in peripheral blood (PBMSC) of rabbits. For mobilization, granulocyte-colony-stimulating factor 30 µg/(kg·d) was injected into New Zealand White rabbits subcutaneously for 6 d, then the PBMSC were isolated from peripheral blood of rabbits by density gradient centrifugation and adhesive culture. The morphology of cell proliferation was observed by microscopy, the proliferative curve of cells was drawn. The phenotypes of PBMSC were detected by flow cytometry, the differential capability of PBMSC into osteocytes, chondrocytes and adipocytes was identified. The results showed that the morphology of subcultured PBMSC were spindle or polygonal shaped, and cell population doubling time was 37.4 h. The isolated PBMSC expressed mesenchymal marker CD29, but not expressed hematopoietic marker CD14. Under specific induction conditions, PBMSC demonstrated multipotency to differentiate into osteocytes, chondrocytes and adipocytes. It is concluded that PBMSC are successfully isolated from peripheral blood and cultured, and their multipotential capability of differentiation into osteocytes, chondrocytes and adipocytes are verified.
Insights
Researchers successfully isolated multipotential mesenchymal stem cells from rabbit peripheral blood. These cells, mobilized by G-CSF, can differentiate into bone, cartilage, and fat cells, confirming their regenerative potential.
Area of Science:
- Stem Cell Biology
- Regenerative Medicine
- Animal Models
Background:
- Mesenchymal stem cells (MSCs) are crucial for tissue repair and regeneration.
- Peripheral blood is an accessible source of stem cells, but their isolation and characterization require specific protocols.
- Rabbit models are valuable for preclinical studies in regenerative medicine.
Purpose of the Study:
- To isolate and characterize multipotential mesenchymal stem cells from rabbit peripheral blood (PBMSC).
- To verify the differentiation capacity of PBMSC into osteocytes, chondrocytes, and adipocytes.
- To establish a method for obtaining functional MSCs from peripheral blood in rabbits.
Main Methods:
- Mobilization of PBMSC using subcutaneous granulocyte-colony-stimulating factor (G-CSF) in New Zealand White rabbits.
- Isolation of PBMSC via density gradient centrifugation and adhesive culture.
- Phenotypic analysis using flow cytometry and assessment of differentiation potential under specific induction conditions.
Main Results:
- Subcultured PBMSC exhibited spindle or polygonal morphology with a population doubling time of 37.4 hours.
- Isolated PBMSC expressed the mesenchymal marker CD29 and lacked the hematopoietic marker CD14.
- PBMSC successfully differentiated into osteocytes, chondrocytes, and adipocytes, demonstrating multipotency.
Conclusions:
- Multipotential mesenchymal stem cells can be successfully isolated and cultured from rabbit peripheral blood.
- The isolated PBMSC possess the capacity to differentiate into osteogenic, chondrogenic, and adipogenic lineages.
- This study validates PBMSC as a promising cell source for regenerative applications in rabbits.
