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Related Experiment Video

Updated: May 13, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Published on: September 2, 2025

Gateway(®) recombinational cloning: a biological operating system.

Federico Katzen¹

¹Invitrogen Corp., 1600 Faraday Avenue, Carlsbad, CA 92008, USA. federico.katzen@invitrogen.com.

Expert Opinion on Drug Discovery

|March 15, 2013

Summary

This summary is machine-generated.

The Gateway cloning system offers a highly efficient and adaptable method for molecular biology, improving DNA segment transfer between expression platforms. This review discusses its benefits and weaknesses compared to traditional cloning techniques.

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Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

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Last Updated: May 13, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Published on: September 2, 2025

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Area of Science:

Molecular Biology
Biotechnology
Genetics

Background:

Traditional cloning methods present challenges in adaptability, efficiency, and compatibility.
Site-specific recombination systems offer potential solutions for improved cloning workflows.

Purpose of the Study:

To review the key aspects and potential applications of the Gateway cloning system.
To discuss the benefits and weaknesses of the Gateway system in comparison to other cloning technologies.

Main Methods:

Review of existing literature on the Gateway cloning system.
Analysis of the underlying phage lambda site-specific recombination mechanism.
Comparative assessment of Gateway technology against traditional cloning approaches.

Main Results:

The Gateway system provides a high-throughput method for DNA cloning, combining, and transferring.
It maintains the orientation and reading frame of DNA fragments across various expression platforms.
The system addresses limitations of traditional cloning, enhancing adaptability and efficiency.

Conclusions:

The Gateway cloning system represents a significant advancement in molecular biology techniques.
Its efficiency, adaptability, and compatibility make it a valuable tool for diverse research applications.
Understanding its benefits and weaknesses is crucial for selecting optimal cloning strategies.