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A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
11:40

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Published on: January 30, 2018

Effect of X-ray irradiation on expression of organic anion transporting polypeptides

Kai Zhan1, Juan Yao, Jiujiu Huang

  • 1College of Life Science, South China Agricultural University , Guangzhou, Guangdong Province.

International Journal of Radiation Biology
|March 15, 2013
PubMed
Abstract

Insights

Radiation therapy can increase the expression of organic anion transporting polypeptides (OATP) in cancer cells, enhancing targeted drug delivery for improved anti-tumor treatment when combined with chemotherapy.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Organic anion transporting polypeptides (OATP) influence drug concentrations in target tissues.
  • OATP transporters are potential targets for optimizing anti-cancer drug efficacy.

Purpose of the Study:

  • To investigate if radiation alters OATP expression in cancer cells.
  • To evaluate OATP as a potential drug target for combined radiotherapy and anti-tumor treatment.

Main Methods:

  • Irradiation of HepG2 and MCF7 cancer cell lines with X-rays.
  • Assessment of expression levels for six OATP transporters post-irradiation.

Main Results:

  • Irradiation altered the expression of several OATP transporters.
  • OATP1A2 expression significantly increased in MCF7 cells after irradiation.
  • Combined radiation and OATP1A2 substrate (methotrexate) showed enhanced anti-tumor effects.

Conclusions:

  • Radiation may upregulate OATP uptake transporters and downregulate efflux transporters.
  • OATP transporters represent a novel target for anti-neoplastic agent delivery in conjunction with radiotherapy.

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