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Expression of P-selectin (CD62P) on platelets after thrombin and ADP in hypotrophic and healthy, full-term newborns
Alicja Wasiluk1, Halina Kemona, Maria Mantur
1Department of Neonatology, Medical University of Bialystok, 15-276 Bialystok, ul. MC. Sklodowskiej-Curie 24a, Poland. awasiluk@umb.edu.pl
Insights
Hypotrophic newborns show increased platelet activation compared to healthy infants. This suggests potential hemostasis disorders in growth-restricted infants, as indicated by P-selectin expression.
Area of Science:
- Neonatal Medicine
- Hematology
- Pediatric Research
Background:
- Hypotrophic newborns often exhibit lower platelet counts, raising concerns about hemostasis disorders.
- P-selectin (CD62P) expression on platelets serves as a marker for platelet activation.
- Platelet reactivity can be assessed using strong (thrombin) and weak (ADP) activators.
Purpose of the Study:
- To investigate platelet activation in hypotrophic newborns compared to healthy controls.
- To determine if gender influences P-selectin expression in hypotrophic newborns.
Main Methods:
- Studied 48 hypotrophic newborns (weight < 10th centile) and 55 healthy term newborns.
- Examined P-selectin (CD62P) expression on platelets in basal state and after stimulation with thrombin or ADP.
Main Results:
- Hypotrophic newborns displayed significantly higher P-selectin expression (4.21%) versus controls (2.88%).
- Thrombin stimulation showed a marked increase in CD62P expression in hypotrophic infants (31.5%) compared to controls (12.5%, p < 0.001).
- ADP stimulation also revealed higher CD62P expression in hypotrophic infants (9.54%) versus controls (4.5%, p = 0.002).
Conclusions:
- Hypotrophic newborns exhibit enhanced platelet activation compared to healthy term newborns.
- Platelet activation differences are not influenced by gender in the studied groups.
Background:
Hypotrophic newborns in comparison with eutrophic newborns demonstrate a reduced blood platelet count and therefore may have haemostasis disorders. Expression of P-selectin (CD62P) on the surface of platelets is a marker of stimulated, activated blood platelets. The ability of platelets to react can be determined after the addition of the following activators: strong (thrombin) and weak (ADP).
Materials And Methods:
We studied 48 hypotrophic newborns, 25 females and 23 males, weighing less than the 10th centile and 55 healthy, full-term newborns, 25 females and 30 males. Expression of CD62P on the surface of platelets was examined in the native state, after the addition of thrombin or ADP.
Results:
Hypotrophic newborns exhibited almost double the percentage of platelets expressing CD62P compared with the control group, 4.21% versus 2.88%. After the addition of thrombin, the percentage was 31.5% versus 12.5%, p < 0.001, whereas after the addition of ADP, the percentage was 9.54% versus 4.5%, p = 0.002.
Conclusions:
Hypotrophic newborns are capable of greater platelet activation in comparison with healthy term newborns. However, gender does not affect the expression of P-selectin.
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