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Related Experiment Video

Updated: May 13, 2026

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach

Published on: December 1, 2011

Trofile HIV co-receptor usage assay.

Andrew J Low¹, Rachel A McGovern, P Richard Harrigan

¹20/48 Nelson Street, Annandale, NSW 2038, Australia +0 450 010 207 ; +0 604 806 9044 andrewjohnlow@gmail.com.

Expert Opinion on Medical Diagnostics

|March 15, 2013

Summary

This summary is machine-generated.

The Monogram Trofile assay is the current standard for determining HIV tropism, essential for guiding CCR5 antagonist therapy. However, improvements in speed, cost, and test stability are needed for broader clinical application.

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Last Updated: May 13, 2026

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Published on: December 27, 2010

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Area of Science:

Virology
Immunology
Pharmacology

Background:

CCR5 antagonists offer new HIV treatment options.
These drugs are ineffective against HIV variants using the CXCR4 co-receptor.
Accurate HIV co-receptor tropism testing is crucial before starting CCR5 antagonist therapy.

Purpose of the Study:

To critically review the Monogram Trofile assay for HIV tropism determination.
Evaluate the assay's performance based on available literature and data.

Main Methods:

Literature review of published studies on the Monogram Trofile assay.
Analysis of available data regarding assay performance and clinical utility.

Main Results:

Monogram Trofile is the de facto standard HIV tropism assay.
Significant limitations exist, including speed, cost, and availability.
The assay is non-quantitative, requires high viral loads, and yields results with suboptimal biological stability.

Conclusions:

Technical limitations of the Monogram Trofile assay may impact CCR5 antagonist therapy use.
Despite limitations, it will likely remain the primary tropism diagnostic in the short term.
Development of more practical and accurate HIV tropism assays is anticipated.