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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
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Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
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Arrayed antibody library technology for therapeutic biologic discovery.

Cornelia A Bentley1, Omar A Bazirgan, James J Graziano

  • 1Fabrus Inc., La Jolla, CA, USA.

Methods (San Diego, Calif.)
|March 15, 2013
PubMed
Summary

Arrayed protein libraries enable screening-based antibody discovery for challenging targets like cell surface proteins. This approach expands antibody functionality beyond high-affinity antagonism, overcoming limitations of traditional selection methods.

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Area of Science:

  • Biochemistry
  • Immunology
  • Drug Discovery

Background:

  • Traditional antibody discovery relies on competitive selection, limiting targets to easily purified proteins.
  • This method primarily yields antibodies for high-affinity antagonism, restricting functional diversity.
  • Difficult-to-purify targets, such as membrane proteins, remain largely inaccessible to conventional discovery techniques.

Purpose of the Study:

  • To review advancements in arrayed protein libraries for screening-based antibody discovery.
  • To highlight the potential of single-molecule-per-well libraries for identifying novel therapeutic antibodies.
  • To discuss the expansion of antibody discovery to previously inaccessible targets and epitopes.

Main Methods:

  • Development of arrayed protein libraries with single-molecule-per-well format.
  • Screening of these libraries using multiplex formats.
  • Application of screening against both purified antigens and cell-surface-expressed targets.

Main Results:

  • Demonstrated feasibility of screening-based discovery using arrayed libraries.
  • Successful identification of antibodies against challenging targets like GPCRs and ion channels.
  • Enabled discovery of antibodies targeting epitopes previously considered inaccessible.

Conclusions:

  • Arrayed protein libraries offer a powerful alternative to selection-based antibody discovery.
  • This technology significantly broadens the scope of therapeutic targets, including complex membrane proteins.
  • Facilitates the development of diverse antibody functionalities beyond antagonism.