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Related Concept Videos

Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Autophagic Cell Death01:18

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and pro-apoptotic...
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

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Related Experiment Video

Updated: May 13, 2026

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

Autophagy in stem cells.

Jun-Lin Guan1, Anna Katharina Simon, Mark Prescott

  • 1Division of Molecular Medicine, Department of Internal Medicine and Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA. lguan@umich.edu

Autophagy
|March 15, 2013
PubMed
Summary
This summary is machine-generated.

Autophagy, a cellular recycling process, is vital for stem cell maintenance and function. Recent studies reveal its critical role in embryonic, tissue, and cancer stem cells, impacting homeostasis and differentiation.

Keywords:
autophagycancer stem cellsembryonic stem cellstissue stem cells

More Related Videos

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
09:00

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

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Last Updated: May 13, 2026

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
09:00

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

Area of Science:

  • Cell Biology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Autophagy is a fundamental cellular process for degrading and recycling cytoplasmic components via lysosomes.
  • Dysfunctional autophagy is linked to various diseases, including cancer and neurodegenerative disorders.
  • Stem cells possess unique self-renewal and differentiation capabilities essential for development and tissue repair.

Purpose of the Study:

  • To review the current understanding of autophagy mechanisms and regulation in different types of stem cells.
  • To explore the role of autophagy in maintaining stem cell homeostasis, expansion, and differentiation.
  • To identify knowledge gaps and future research directions in autophagy and stem cell biology.

Main Methods:

  • Comprehensive literature review of recent studies on autophagy in stem cells.
  • Analysis of findings from knockout mice models to elucidate gene functions.
  • Synthesis of current knowledge on autophagy's role in embryonic, tissue, and cancer stem cells.

Main Results:

  • Autophagy plays a crucial role in the quality control and homeostasis of stem cells.
  • Specific autophagy genes and pathways significantly regulate stem cell maintenance, expansion, and differentiation.
  • Emerging research highlights the importance of autophagy in embryonic stem cells, hematopoietic stem cells, and cancer stem cells.

Conclusions:

  • Autophagy is indispensable for stem cell function and integrity.
  • Further research into autophagy's role in stem cells is critical for understanding development and disease.
  • Understanding autophagy in stem cells opens new avenues for therapeutic interventions.