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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: May 13, 2026

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

Published on: February 2, 2024

High-throughput flow cytometry for drug discovery.

Bruce S Edwards¹, Susan M Young, Matthew J Saunders

¹Cytometry and Department of Pathology, CRTC, UNM HSC, MS08-4630, Albuquerque, NM 87131, USA +1 505 272 6206 ; +1 505 272 6695 ; bedwards@salud.unm.edu.

Expert Opinion on Drug Discovery

|March 16, 2013

Summary

This summary is machine-generated.

High-throughput flow cytometry accelerates data acquisition and reduces sample volume for bioassays. This enables efficient screening of compounds and analysis of complex biological systems.

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Area of Science:

Biotechnology
Cell Biology
Drug Discovery

Background:

Flow cytometry is a powerful tool for analyzing cells and biomaterials.
Traditional flow cytometry can be limited by data acquisition speed and sample volume requirements.

Purpose of the Study:

To introduce a novel high-throughput flow cytometry approach.
To enhance efficiency in data acquisition and sample analysis for bioassays.
To enable rapid screening of compounds and exploration of biological diversity.

Main Methods:

A many-samples/one-file data acquisition strategy was developed.
The method was applied to cell- and microsphere-based bioassays in 96- and 384-well formats.
High-content multiparametric analysis was utilized for assay multiplexing.

Main Results:

Dramatically increased data acquisition speed.
Reduced aspirated sample volume to as little as 2 μl/well.
Successfully screened tens-of-thousands of compounds, identifying novel bioactive structures.
Enabled integrated assessment of biologic selectivity and specificity in primary screens.

Conclusions:

High-throughput flow cytometry offers a powerful and efficient tool for biological and chemical research.
The novel approach facilitates automated analysis and group-wise comparison of multisample data.
Advances in flow cytometry are crucial for probing complex biological systems and accelerating drug discovery.