Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surface substance loss of subsurface bovine enamel lesions after different steps of the resinous infiltration technique: a 3D topography analysis.

Odontology·2011
Same author

Expression of IL-23/Th17 pathway in a murine model of Coxsackie virus B3-induced viral myocarditis.

Virology journal·2011
Same author

Extracellular matrix peptides of Artemia cyst shell participate in protecting encysted embryos from extreme environments.

PloS one·2011
Same author

Therapeutic effect of carboxymethylated and quanternized chitosan on insulin resistance in high-fat-diet-induced rats and 3T3-L1 adipocytes.

Journal of biomaterials science. Polymer edition·2011
Same author

Role of reactive oxygen species in triptolide-induced apoptosis of renal tubular cells and renal injury in rats.

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban·2011
Same author

Chlorination and ortho-acetoxylation of 2-arylbenzoxazoles.

Organic & biomolecular chemistry·2011
Same journal

A dual-engineered chondrocyte-targeting tetrahedral DNA-based nanoparticle for improved delivery of metformin and alleviation in osteoarthritis.

Biomaterials·2026
Same journal

Machine learning-guided design of cooperative multi-size hydrogel microspheres for osteoarthritis therapy.

Biomaterials·2026
Same journal

Cationic nanotrap curbs UVB-induced cutaneous photodamage via exosomal cfNA capture.

Biomaterials·2026
Same journal

A protein-initiated polymerization cascade enables a self-eliminating powder tissue adhesive for diabetic ulcer repair.

Biomaterials·2026
Same journal

Oral colon-targeted micro-nano formulation engineered in microfluid for synergistic therapy of inflammatory bowel disease.

Biomaterials·2026
Same journal

Manganese@Gold cluster-coordinated covalent organic frameworks-based artificial metalloenzymes with cascade biocatalysis and amplified systemic stimulation to combat malignant tumor metastasis.

Biomaterials·2026
See all related articles

Related Experiment Video

Updated: May 13, 2026

Interlinked Macroporous 3D Scaffolds from Microgel Rods
07:32

Interlinked Macroporous 3D Scaffolds from Microgel Rods

Published on: June 16, 2022

Dynamic tissue engineering scaffolds with stimuli-responsive macroporosity formation.

Li-Hsin Han1, Janice H Lai, Stephanie Yu

  • 1Department of Orthopaedic Surgery, Stanford University, Stanford, CA 94305, USA.

Biomaterials
|March 16, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed dynamic hydrogels for tissue engineering with tunable macroporosity. This breakthrough enhances cell delivery, proliferation, and tissue regeneration by controlling pore size during culture.

More Related Videos

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size
13:46

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size

Published on: October 17, 2016

Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo
12:19

Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo

Published on: July 1, 2013

Related Experiment Videos

Last Updated: May 13, 2026

Interlinked Macroporous 3D Scaffolds from Microgel Rods
07:32

Interlinked Macroporous 3D Scaffolds from Microgel Rods

Published on: June 16, 2022

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size
13:46

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size

Published on: October 17, 2016

Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo
12:19

Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo

Published on: July 1, 2013

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Macropores in tissue engineering scaffolds are vital for vascularization, cell proliferation, and tissue regeneration.
  • Current macroporous scaffold fabrication methods often lack control over porosity during cell culture and use non-physiological conditions, leading to poor cell seeding.
  • Dynamic control over scaffold porosity is needed to optimize cellular processes throughout tissue development.

Purpose of the Study:

  • To develop stimuli-responsive hydrogel scaffolds with tunable macroporosity for tissue engineering.
  • To investigate the use of gelatin, alginate, and hyaluronic acid as stimuli-responsive porogens for dynamic pore formation.
  • To evaluate the impact of dynamic pore formation on chondrocyte behavior and tissue formation.

Main Methods:

  • Fabrication of dynamic hydrogels using stimuli-responsive porogens (gelatin, alginate, hyaluronic acid).
  • Sequential stimulation (temperature, EDTA, hyaluronidase) to induce sequential macropore formation.
  • Encapsulation of bovine chondrocytes within alginate porogens for cell delivery and assessment of cell viability, proliferation, and tissue formation.

Main Results:

  • Confirmed sequential pore formation using SEM imaging in response to specific stimuli at defined time points.
  • Demonstrated effective cell release from alginate porogens with high chondrocyte viability.
  • Observed significantly increased chondrocyte proliferation, spreading, and enhanced production of type II and X collagen.

Conclusions:

  • Developed a novel platform for creating stimuli-responsive scaffolds with dynamic, tunable macroporosity.
  • This approach significantly improves cell delivery, proliferation, and extracellular matrix production in engineered tissues.
  • The platform offers a valuable tool for tissue engineering and fundamental studies on cell-niche interactions.