Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mutations in Microorganisms01:18

Mutations in Microorganisms

Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
Evolution of Microbial Genome01:08

Evolution of Microbial Genome

Microbial genome evolution is a highly dynamic process shaped by continual gene gain and loss across species and strains. This genomic flexibility allows microorganisms to adapt rapidly to environmental pressures and interactions with other organisms. Central to understanding this diversity is the distinction between the core and pan genomes.The core genome comprises the genes shared by all sampled strains of a species, representing essential functions needed for fundamental cellular processes.
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Mutations01:35

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Mutations01:39

Mutations

Overview
Mutations01:39

Mutations

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis, crystal structure, and optical properties of MgO-doped jeanbandyite for optoelectronic applications.

Dalton transactions (Cambridge, England : 2003)·2026
Same author

Clonally related <i>Escherichia coli</i> ST69 causing neonatal sepsis in preterm siblings born 1 year apart.

ASM case reports·2026
Same author

Sphingosine kinase-2 inhibition promotes immunogenic differentiation of myeloid-derived suppressor cells through an Acetyl-CoA carboxylase-phosphatidylcholine axis.

Nature communications·2026
Same author

Molecular Insights into the Selective Ion Permeation and Confined Water Behavior in a Transmembrane d/l-α Cyclic Peptide Nanotube.

The journal of physical chemistry. B·2026
Same author

Ultraslow conformational dynamics and catch bond formation of a bacterial adhesin revealed by a single-domain variant of FimH.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

VancObituary: A Summary of a Life Well Lived and a Death Well Timed.

Journal of the Pediatric Infectious Diseases Society·2026

Related Experiment Video

Updated: May 13, 2026

Following the Dynamics of Structural Variants in Experimentally Evolved Populations
04:52

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Published on: February 3, 2023

Microbial variome database: point mutations, adaptive or not, in bacterial core genomes.

Sujay Chattopadhyay1, Fred Taub, Sandip Paul

  • 1Department of Microbiology, University of Washington, Seattle, USA. sujayc@u.washington.edu

Molecular Biology and Evolution
|March 16, 2013
PubMed
Summary

Researchers developed microbial variome databases to analyze bacterial genetic variations. These resources aid in understanding pathogenesis and identifying targets for new antibacterial drugs.

Keywords:
adaptive evolutiondatabasemicrobial variomenucleotide polymorphisms

More Related Videos

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
06:03

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Published on: September 20, 2016

Related Experiment Videos

Last Updated: May 13, 2026

Following the Dynamics of Structural Variants in Experimentally Evolved Populations
04:52

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Published on: February 3, 2023

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
06:03

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Published on: September 20, 2016

Area of Science:

  • Microbiology
  • Genomics
  • Molecular Evolution

Background:

  • Understanding bacterial genetic differences is key for pathogenesis research and developing new therapeutics.
  • Existing studies lack large, well-defined strain collections for genome-wide association studies, hindering identification of adaptive genes and polymorphisms.

Purpose of the Study:

  • To create a "microbial variome"—a species-specific database of genomic variations using molecular evolutionary analysis.
  • To address limitations in identifying genes under positive selection and adaptive polymorphisms.

Main Methods:

  • Developed prototype variome databases for Escherichia coli and Salmonella enterica subspecies enterica.
  • Included point mutation data for core protein-coding genes from 22 E. coli and 17 S. enterica strains.
  • Implemented single- and multiple-field sorting, filtering, and searching capabilities for gene variability data.

Main Results:

  • Presented publicly available prototype variome databases for E. coli and S. enterica.
  • Databases contain detailed genetic variation data, including nucleotide polymorphisms.
  • Facilitates analysis of potential adaptive significance of genetic variations.

Conclusions:

  • Microbial variome databases are essential resources for advancing research in bacterial pathogenesis and drug discovery.
  • These databases will significantly aid experimental research, clinical diagnostics, epidemiology, and pathogen control efforts.