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Updated: May 13, 2026
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Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
Published on: June 20, 2018
Ex vivo culture and separation of functional renal cells
Andrew T Bruce1, Kelly I Guthrie, Rusty Kelley
1Tengion Inc., Winston-Salem, NC, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 16, 2013
Abstract:
The following methods outline the procedures for isolating primary renal cells from kidney tissue via enzymatic digestion, followed by their culture, harvest, and then fractionation of renal subpopulations from primary culture. The current methods describe procedures to sub-fractionate biologically active cells that have been used to treat and stabilize renal function in models of chronic kidney disease (Kelley et al. Am J Physiol Renal Physiol 299(5):F1026-F1039, 2010).
