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Related Experiment Video

Updated: May 13, 2026

Decellularization and Recellularization of Whole Livers
09:24

Decellularization and Recellularization of Whole Livers

Published on: February 4, 2011

Human liver bioengineering using a whole liver decellularized bioscaffold.

Pedro M Baptista1, Dipen Vyas, Emma Moran

  • 1Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 16, 2013
PubMed
Summary

Human liver bioengineering is now more feasible due to advances in whole organ decellularization. This chapter details steps for creating a bioengineered liver scaffold using decellularized ferret livers and human cells.

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Area of Science:

  • Regenerative Medicine
  • Biotechnology
  • Organ Engineering

Background:

  • Significant advancements in whole organ decellularization techniques have been achieved.
  • Organ bioengineering is becoming increasingly feasible.

Purpose of the Study:

  • To detail the necessary steps in human liver bioengineering.
  • To provide a comprehensive overview of liver scaffold creation.

Main Methods:

  • Whole organ decellularization of ferret livers using detergent perfusion.
  • Isolation of human liver progenitor and endothelial cells.
  • Recellularization of the liver bioscaffold in a perfusion bioreactor.

Main Results:

  • Successful decellularization of ferret liver tissue.

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Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device
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Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

Published on: August 10, 2015

Related Experiment Videos

Last Updated: May 13, 2026

Decellularization and Recellularization of Whole Livers
09:24

Decellularization and Recellularization of Whole Livers

Published on: February 4, 2011

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat
13:27

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

Published on: October 6, 2018

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device
09:38

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

Published on: August 10, 2015

  • Isolation of viable human liver progenitor and endothelial cells.
  • Demonstration of recellularization within a bioreactor system.

    • Conclusions:

    • Human liver bioengineering is a promising area of research.
    • The described methods provide a framework for future liver scaffold development.
    • Further research is needed to optimize cell integration and vascularization.