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The Intrinsic Apoptotic Pathway

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Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

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Related Experiment Video

Updated: May 13, 2026

Examining BCL-2 Family Function with Large Unilamellar Vesicles

Examining BCL-2 Family Function with Large Unilamellar Vesicles

Published on: October 5, 2012

Bcl-3 regulates UVB-induced apoptosis.

Ingrid García¹, Gabriela Cosío, Floria Lizárraga

¹National Institute of Cancerology, Av. San Fernando 22 Tlalpan, 14080 Mexico, Mexico.

|March 16, 2013

Summary

This summary is machine-generated.

B cell leukemia-3 (Bcl-3) protects cells from apoptosis by regulating both intrinsic and extrinsic pathways. Silencing Bcl-3 accelerates caspase activation and impacts DNA repair proteins.

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Area of Science:

Cell Biology
Molecular Biology
Cancer Research

Background:

B cell leukemia-3 (Bcl-3) is recognized as an anti-apoptotic gene.
The precise mechanisms by which Bcl-3 regulates apoptosis remain unclear.
Understanding Bcl-3's role is crucial for developing targeted cancer therapies.

Purpose of the Study:

To elucidate the specific role of Bcl-3 in apoptosis.
To investigate the impact of Bcl-3 silencing on apoptotic pathways.
To identify key proteins regulated by Bcl-3 during apoptosis induction.

Main Methods:

Evaluating the effect of Bcl-3 silencing on protein expression.
Analyzing extrinsic and intrinsic apoptotic pathways.
Utilizing ultraviolet light B (UVB) to induce DNA damage.

Main Results:

Bcl-3 silencing accelerated caspase-3, caspase-8, and caspase-9 activation.
Increased tBid mitochondrial content was observed in Bcl-3 silenced cells.
Diminished levels of p53 and DNA-PK were noted in Bcl-3 silenced cells.

Conclusions:

Bcl-3 plays a protective role against apoptosis.
Bcl-3 regulates both extrinsic and intrinsic apoptotic pathways.
Bcl-3 influences DNA repair mechanisms through proteins like p53 and DNA-PK.