Related Experiment Video
Updated: May 13, 2026
Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
Published on: January 13, 2016
Proteomics-based expression library screening - a platform technology for rapid discovery of pathogen-specific
Indira T Kudva1, Stephen B Calderwood, Manohar John
1Massachusetts General Hospital, Division of Infectious Diseases, Boston, MA 02114, USA +1 781 244 4505 ; micronome@yahoo.com.
Background:
The development of new management modalities is imperative for reducing the global burden of infectious diseases.
Objective:
To develop a platform technology for rapid definition of comprehensive pathogen immunoproteomes (the repertoire of microbial proteins that elicit and interact with host immune responses).
Methods:
Standard molecular biology techniques were coupled with cutting-edge proteomics to develop proteomics-based expression library screening (PELS).
Results:
Proof of principle of PELS was demonstrated by defining a comprehensive immunoproteome of the human gastrointestinal pathogen, Escherichia coli O157:H7, in bovine reservoirs in just 3 weeks.
Conclusions:
PELS, an immunoproteomics-based platform technology, is a rapid and inexpensive alternative to protein/antigen arrays/chips. It is applicable to any parasitic, fungal, viral or bacterial pathogen (or commensal) that: has a sequenced genome; can be cultured in the laboratory; and elicits host humoral immune responses during the process of infection/disease.
