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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

Cell-based assays for high-throughput screening.

Frank H Büttner¹

¹Boehringer Ingelheim Pharma GmbH & Co. KG, Department of Lead Discovery, Building K91, Birkendorferstr. 65, 88397 Biberach an der Riss, Germany. Frank.Buettner@BC.Boehringer-Ingelheim.com.

Expert Opinion on Drug Discovery

|March 19, 2013

Summary

This summary is machine-generated.

This report summarizes the

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Area of Science:

Pharmaceutical Sciences
Biotechnology
Drug Discovery

Background:

The fifth World Pharmaceutical Congress featured six concurrent conferences.
This report focuses on the 'Cell-based assays for HTS' conference.
Key topics included screening assay technologies, cells as reagents, 3D cell culture, and stem cell applications.

Purpose of the Study:

To review advancements in cell-based assays for high-throughput screening (HTS).
To highlight new technologies and strategies in drug discovery screening.
To discuss the role of cells, including stem cells and 3D cultures, as biological reagents.

Main Methods:

Presentations covered technologies like open microscopy, cell culture arrays, and aptamer technology.
Success stories using enzyme fragment complementation assays (e.g., InteraX, DiscoveRx) were shared.
Discussions included Cell Sensor Technology, dual luciferase assays for GPCRs, and ion channel assay comparisons.

Main Results:

New technologies for cell-based screening were showcased.
The utility of enzyme fragment complementation and other assay technologies in drug discovery was demonstrated.
The potential of 3D cell cultures and stem cells for drug screening was emphasized.

Conclusions:

Cell-based assays are rapidly evolving with new technologies.
Overcoming challenges in using cells as reagents is crucial for HTS success.
Stem cells and 3D cultures offer promising avenues for future drug discovery efforts.