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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: May 13, 2026

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Biomarker discovery and compound evaluation using two-hybrid proteomic systems.

Marc E Gillespie1

  • 1Department of Pharmaceutical Sciences, College of Pharmacy & Allied Health Professions, St. John's University, 8000 Utopia Parkway, Jamaica, New York 11439, USA.

Expert Opinion on Drug Discovery
|March 19, 2013
PubMed
Summary

The yeast two-hybrid system offers an accessible proteomic approach for identifying protein interactions and cellular pathways. This cost-effective method enables researchers to analyze therapeutic compound effects, even with limited resources.

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Related Experiment Videos

Last Updated: May 13, 2026

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
14:23

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Advanced proteomic technologies often demand significant expertise and financial investment, limiting accessibility for many research labs and smaller companies.
  • Proteomic approaches are valuable for pilot studies, biomarker discovery, and analyzing therapeutic compound effects.

Purpose of the Study:

  • To highlight the utility of the yeast two-hybrid system as an affordable and accessible proteomic technology.
  • To demonstrate how this system can identify protein interactions and construct local protein networks for pathway analysis.

Main Methods:

  • Utilizes the yeast two-hybrid (Y2H) system, a widely available and cost-effective proteomic technique.
  • Employs standard microbiological equipment and commercially available cDNA libraries.
  • Screens rapidly generate data on protein-protein interactions.

Main Results:

  • Identifies protein interactions crucial for constructing small, local protein networks.
  • Enables the inference of larger cellular pathways affected by therapeutic compounds.
  • Facilitates the focus on critical cellular pathways and molecular targets.

Conclusions:

  • The yeast two-hybrid system provides a practical and economical solution for proteomic analysis, particularly for smaller research groups.
  • It empowers researchers to investigate complex biological questions and therapeutic mechanisms using established molecular assays and extensive databases.