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Protein biomarker quantification by mass spectrometry.

Joanna M Hunter1, Eustache Paramithiotis

  • 1Department of Protein Analysis, Caprion Proteomics, Inc., 7150 Alexander-Fleming, Montreal, Quebec, Canada, USA.

Expert Opinion on Medical Diagnostics
|March 19, 2013
PubMed
Summary

Multiple reaction monitoring mass spectrometry (MRM-MS) is emerging as a powerful technique for quantifying protein biomarkers in clinical diagnostics. This method offers precise analysis for biomarker panels, paving the way for advanced biomarker tests.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • The quantification of protein biomarkers is crucial for developing accurate diagnostic tests.
  • Multiple reaction monitoring mass spectrometry (MRM-MS), initially used for small molecules, is now being adapted for peptide quantification.
  • There is growing interest in applying MRM-MS for protein biomarker assays.

Purpose of the Study:

  • To summarize MRM-MS techniques for quantifying protein biomarkers and biomarker panels.
  • To review the clinical applications of MRM-MS in biomarker testing.
  • To explore future directions for MRM-MS in biomarker qualification and verification.

Main Methods:

  • A literature survey was conducted on the use of MRM-MS for quantifying protein biomarker panels.
  • The summary includes techniques for protein biomarker quantification and clinical applications.
  • The analytical characteristics of MRM-MS relevant to clinical laboratory assays were considered.

Main Results:

  • MRM-MS is increasingly adopted for protein biomarker quantification, including panels.
  • The technique is well-suited for adaptation to peptide analysis.
  • Current literature supports the application of MRM-MS in clinical settings.

Conclusions:

  • MRM-MS is a promising approach for protein biomarker quantification and the development of biomarker tests.
  • Future work should focus on the qualification and verification of candidate protein biomarkers using MRM-MS.
  • The analytical precision of MRM-MS makes it ideal for clinical laboratory biomarker assays.