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Related Experiment Videos

A method for counting leukocytes in filtered components.

T J Greenwalt1, C M Allen

  • 1Hoxworth Blood Center, University of Cincinnati Medical Center, OH.

Transfusion
|May 1, 1990
PubMed
Summary
This summary is machine-generated.

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A new hemacytometer method accurately quantifies residual white blood cells (WBCs) in blood components. This simple technique provides reliable quality control for leukocyte-depleted products, preventing alloimmunization.

Area of Science:

  • Blood banking and transfusion medicine
  • Hematology
  • Quality control in medical laboratories

Background:

  • Leukocyte-depleted blood components are increasingly utilized in clinical practice.
  • Accurate quantification of residual white blood cells (WBCs) is crucial for quality assurance.
  • Established methods for WBC counting below 100 cells/microL lack accuracy or are complex.

Purpose of the Study:

  • To develop and validate a simple, accurate method for quantifying low levels of residual WBCs.
  • To establish a reliable quality control measure for leukocyte-reduced blood products.
  • To determine the critical WBC level for preventing transfusion-related alloimmunization.

Main Methods:

  • A straightforward hemacytometer procedure was developed using Türks solution.

Related Experiment Videos

  • The method was specifically designed to quantify WBC levels below 100 cells/microL.
  • Recovery rates were assessed at low WBC concentrations.

    • Main Results:

    • The described hemacytometer method demonstrated 87-93% recovery for WBC levels below 40 cells/microL.
    • This technique offers a significant improvement over electronic counting at low cell densities.
    • The procedure is simple and does not require sophisticated equipment.

    Conclusions:

    • A simple hemacytometer assay provides accurate quantification of residual WBCs in leukocyte-depleted components.
    • This method serves as a valuable tool for quality control in blood banking.
    • Effective monitoring of residual WBCs is essential for preventing alloimmunization in transfusion recipients.