Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Allergic Reactions: Anaphylaxis

Allergic Reactions: Anaphylaxis

Anaphylaxis is a severe, life-threatening hypersensitivity reaction mediated by Immunoglobulin E (IgE) antibodies. When IgE binds to allergens, it triggers the release of mediators– histamine, leukotrienes, and prostaglandins from mast cells and basophils. These mediators cause vasodilation, edema, and inflammation, leading to various symptoms.The primary allergens causing anaphylaxis include food items (e.g., peanuts, shellfish), drugs (e.g., penicillin, asparaginase, corticotropin, heparin),...

Allergic Reactions

Allergic Reactions

Allergic Drug Reactions

Allergic Drug Reactions

Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing numerous...

Asthma-IV: Diagnostic and Management

Asthma-IV: Diagnostic and Management

The diagnosis and management of asthma are comprehensive, encompassing clinical assessments, lung function tests, and pharmacological interventions. Here's an overview:
Clinical Assessment for Asthma:
This is the first step in diagnosing and managing asthma. It includes:

Antibody Structure

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...

Drug Toxicity: Allergic Reactions

Drug Toxicity: Allergic Reactions

Drug-related allergies are immune-mediated responses triggered by the administration of pharmacological agents. These hypersensitivity reactions are classified based on the immune mechanisms involved. The four primary types—Type I, II, III, and IV—are mediated by different immunological pathways and exhibit distinct clinical manifestations.Type I Hypersensitivity/ IgE-Mediated Reactions: Immunoglobulin E (IgE) immediately mediates Type I hypersensitivity reactions. Upon initial exposure to a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Molecular allergen inhibition assay for allergen extract identity, purity, and potency assessment.

The Journal of allergy and clinical immunology·2026

Same author

A Human Monoclonal Antibody Displays Promiscuous Binding to Multiple Type 1 nsLTP Allergens.

Journal of investigational allergology & clinical immunology·2026

Same author

Successful evaluation and management of insulin hypersensitivity reactions.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology·2025

Same author

Identifying immunodominant proteins in mouse urine.

The Journal of allergy and clinical immunology·2025

Same author

Engrafted NSG-SGM3 humanized mice spontaneously produce human immunoglobulins including IgE.

Frontiers in immunology·2025

Same author

Effective pest control alone reduces mouse allergens in low-income housing.

The journal of allergy and clinical immunology. Global·2025

Same journal

Evaluation and management of pulmonary nodules: state-of-the-art and future perspectives.

Expert opinion on medical diagnostics·2013

Same journal

Biomarkers in IgA nephropathy: relationship to pathogenetic hits.

Expert opinion on medical diagnostics·2013

Same journal

The molecular landscape of diffuse glioma and prospects for biomarker development.

Expert opinion on medical diagnostics·2013

Same journal

Difficulties and barriers in diagnosing Fabry disease: what can be learnt from the literature?

Expert opinion on medical diagnostics·2013

Same journal

Promising biomarkers for systemic lupus erythematosus.

Expert opinion on medical diagnostics·2013

Same journal

Challenges of COPD diagnosis.

Expert opinion on medical diagnostics·2013

See all related articles

Search research articles

Related Experiment Video

Updated: May 13, 2026

Basophil Activation Test for Allergy Diagnosis

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

Diagnostic tests for human allergic disease.

Robert G Hamilton¹

¹Professor of Medicine and Pathology, Johns Hopkins University School of Medicine and Director, Johns Hopkins Dermatology, Allergy and Clinical Immunology Reference Laboratory.

Expert Opinion on Medical Diagnostics

|March 19, 2013

Summary

This summary is machine-generated.

Diagnosing allergic disease relies on history, skin tests, and blood tests for IgE antibody. While both methods have limitations, advancements in in vitro IgE testing offer improved accuracy for allergy diagnosis.

More Related Videos

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

Published on: March 3, 2023

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Published on: April 21, 2019

Related Experiment Videos

Last Updated: May 13, 2026

Basophil Activation Test for Allergy Diagnosis

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

Published on: March 3, 2023

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Published on: April 21, 2019

Area of Science:

Allergy and Immunology
Diagnostic Medicine

Background:

Allergic disease diagnosis starts with clinical history and physical exams.
Confirmatory tests for immunoglobulin E (IgE) antibody, including skin and blood tests, verify sensitization after allergen exposure.
The diagnostic utility of in vivo (skin) versus in vitro (blood) IgE antibody measures is debated.

Purpose of the Study:

To examine the strengths and limitations of primary diagnostic methods for IgE antibody detection.
To compare in vivo (skin tests, provocation tests) and in vitro (blood tests) IgE antibody assays.
To review factors influencing the accuracy of IgE antibody assays.

Main Methods:

A literature review was conducted.
Diagnostic performance characteristics of IgE antibody assays were examined.
Variables affecting the accuracy of IgE antibody assays were analyzed.

Main Results:

Both clinical history and IgE antibody measures (in vivo and in vitro) are subject to variability and bias.
The relative diagnostic performance of in vivo versus in vitro IgE assays is difficult to determine without a definitive clinical history-based gold standard.
Skin testing has seen limited innovation, while in vitro assays have significantly improved in performance and reagent quality.

Conclusions:

Accurate allergic disease diagnosis requires careful consideration of IgE antibody testing methods.
Advancements in in vitro IgE assays, including point-of-care testing and recombinant allergens, enhance diagnostic capabilities.
Future diagnostic approaches may integrate automation and predictive algorithms for improved allergy assessment.