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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...

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Related Experiment Video

Updated: May 13, 2026

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
12:06

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Published on: February 1, 2017

From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments.

Emiliano Pérez Ipiña1, Silvina Ponce Dawson

  • 1Departamento de Física, FCEN-UBA, and IFIBA, CONICET, Ciudad Universitaria, Pabellón I, (1428) Buenos Aires, Argentina.

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
|March 19, 2013
PubMed
Summary
This summary is machine-generated.

Fluorescence correlation spectroscopy (FCS) can accurately measure effective diffusion coefficients, even when molecular interactions are not extremely fast. Adjusting observation volume or concentrations helps extract more system information.

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Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
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Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

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Last Updated: May 13, 2026

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
12:06

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Published on: February 1, 2017

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
05:56

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

Area of Science:

  • Biophysics
  • Cellular Biology
  • Physical Chemistry

Background:

  • Molecular transport within cells is crucial for biological processes.
  • Diffusion and molecular interactions govern the distribution of molecules.
  • Effective diffusion coefficients describe transport when reactions are faster than diffusion.

Purpose of the Study:

  • Investigate the accuracy of effective diffusion coefficients derived from FCS.
  • Analyze FCS performance across various reaction-to-diffusion time scale ratios.
  • Explore how fitting functions impact coefficient inference.

Main Methods:

  • Utilized Fluorescence Correlation Spectroscopy (FCS).
  • Employed diverse fitting functions for data analysis.
  • Varied the ratio of reaction to diffusion time scales.

Main Results:

  • Effective diffusion coefficients can be inferred with minimal error, even if reactions aren't strictly fast.
  • FCS provides insights into effective diffusion under non-ideal reaction conditions.
  • The study covers a broad spectrum of reaction-to-diffusion time scale ratios.

Conclusions:

  • FCS is a robust technique for measuring effective diffusion coefficients.
  • Adjusting observation volume and molecular concentrations allows for nuanced system analysis.
  • Understanding these parameters enhances the interpretation of molecular transport dynamics.