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Updated: May 13, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Aptamer amplification: divide and signal.

Supriya Pai1, Ana Roberts, Andrew D Ellington

  • 1Graduate Research Assistant University Station, 1, MBB 3.424, 2500 Speedway, Austin, TX 78712, USA.

Expert Opinion on Medical Diagnostics
|March 19, 2013
PubMed
Summary
This summary is machine-generated.

Aptamers, or selected nucleic acids, can be used in amplification assays for sensitive protein detection. While effective, amplification offers few advantages over other formats unless using proximity ligation assays for ultra-sensitive detection.

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Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Aptamers are nucleic acid molecules selected for high-affinity and specific binding to targets.
  • Their properties are comparable to monoclonal antibodies in binding capabilities.

Purpose of the Study:

  • To review aptamer-based amplification assays, a format not previously covered.
  • To highlight the unique utility of aptamers in nucleic acid amplification technologies.

Main Methods:

  • Aptamers are employed as binding reagents detected via methods like polymerase chain reaction (PCR) and rolling circle amplification (RCA).
  • Ligand-induced conformational changes or unique quaternary structures of aptamers can trigger amplification.

Main Results:

  • Aptamer amplification assays enable sensitive detection of target proteins, often in the nanomolar to picomolar range.
  • Proximity ligation assays, utilizing aptamers and sequence amplification, allow detection of extremely low ligand quantities against background.

Conclusions:

  • While aptamer amplification assays offer sensitive detection, advantages over other formats are often limited by aptamer dissociation constants.
  • Proximity ligation assays represent a key exception, demonstrating superior sensitivity through sequence amplification for ligand detection.