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Related Experiment Video

Updated: May 13, 2026

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe

Published on: May 2, 2025

Nedd8 processing enzymes in Schizosaccharomyces pombe.

Jean E O'Donoghue¹, Dawadschargal Bech-Otschir, Ida B Larsen

¹MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.

BMC Biochemistry

|March 19, 2013

Summary

In fission yeast, Uch1 is not the only enzyme that processes the Nedd8 precursor. Deubiquitylating enzymes also play a key role in Nedd8 activation, which is crucial for SCF ligase function.

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Last Updated: May 13, 2026

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Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

The conjugation of ubiquitin-like modifier Nedd8 to cullins is essential for SCF-type ubiquitin ligase activity.
SCF ligases regulate the degradation of various substrates, including cell cycle regulators.
Nedd8, like ubiquitin, is synthesized as an inactive precursor requiring processing for activation.

Purpose of the Study:

To investigate the enzymes responsible for Nedd8 precursor processing in the fission yeast Schizosaccharomyces pombe.
To determine if the Yuh1 orthologue, Uch1, is the sole Nedd8-processing enzyme in S. pombe.

Main Methods:

Comparative analysis of Nedd8 processing in Schizosaccharomyces pombe.
Identification and characterization of enzymes involved in Nedd8 precursor maturation in vivo.

Main Results:

The Yuh1 orthologue, Uch1, is not the exclusive enzyme for Nedd8 precursor processing in S. pombe.
Deubiquitylating enzymes were found to efficiently process the Nedd8 precursor in vivo.

Conclusions:

Nedd8 precursor processing in S. pombe involves multiple enzymes.
A variety of deubiquitylating enzymes contribute to the activation of Nedd8 in vivo.