Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Coagulation01:06

Coagulation

Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
Coagulation01:09

Coagulation

The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tissue factor-related biomarkers during combined androgen blockade in patients with prostate cancer: A brief report.

Journal of thrombosis and thrombolysis·2026
Same author

Plasma heme pool compartmentalization is linked to pathophysiology in Sickle Cell Disease.

PloS one·2026
Same author

Can hyperleukocytosis be caused by a non-hematologic condition? A 10-year retrospective tertiary-care center cohort study.

Annals of medicine·2026
Same author

Astrocytes enable amygdala neural representations supporting memory.

Nature·2026
Same author

Long-term real-world thrombotic and clinical outcomes in polycythemia vera - a hospital-based i2b2 cohort study.

Annals of hematology·2026
Same author

The interaction of fibrinolysis and the complement system in patients with acute pulmonary embolism, treated with ultrasound-assisted catheter-directed thrombolysis.

Thrombosis journal·2026

Related Experiment Video

Updated: May 13, 2026

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
08:01

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

Variability between laboratories performing coagulation tests with identical platforms: a nationwide evaluation

Michael Nagler1, Lucas M Bachmann, Lorenzo Alberio

  • 1Division of Hematology and Central Hematology Laboratory, Luzerner Kantonsspital, Lucerne 16 CH-6000, Switzerland. walter.wuillemin@luks.ch.

Thrombosis Journal
|March 19, 2013
PubMed
Summary
This summary is machine-generated.

Laboratory procedures significantly impact coagulation test results, leading to low reliability between labs. Improving standardization in quantifying coagulation factors is crucial for accurate medical diagnostics.

More Related Videos

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
05:58

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes

Published on: March 22, 2022

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
13:08

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

Related Experiment Videos

Last Updated: May 13, 2026

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
08:01

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
05:58

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes

Published on: March 22, 2022

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
13:08

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

Area of Science:

  • Clinical chemistry
  • Hematology
  • Laboratory medicine

Background:

  • Analytical precision in medical labs is studied, but inter-laboratory variation sources are unclear.
  • This study quantifies variance components in coagulation testing across different labs using identical platforms.

Purpose of the Study:

  • To quantify variance components in coagulation tests performed across different laboratories.
  • To compute intraclass correlation coefficients (ICC) for various coagulation tests.

Main Methods:

  • Eight labs measured fibrinogen twice in 20 subjects using 3 platforms.
  • Single measurements of prothrombin time (PT) and coagulation factors II, V, VII, VIII, IX, X, XI, and XIII were analyzed.
  • ANOVA was used to determine variance components (subject, lab/technician, total).

Main Results:

  • Fibrinogen variability within labs: 0.02-0.04; between labs: 0.006-0.097.
  • ICC for fibrinogen: 0.37-0.66; for PT: 0.19-0.80.
  • ICC for other factors ranged from 0.04 (FII) to 0.93 (FVIII).

Conclusions:

  • Substantial variance attributed to technicians/lab procedures.
  • Low ICCs for several factors, particularly on certain platforms.
  • Highlights the need for enhanced standardization in coagulation factor quantification.