Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 13, 2026

Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement
05:28

Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement

Published on: August 9, 2024

A distance-field based automatic neuron tracing method.

Jinzhu Yang1, Paloma T Gonzalez-Bellido, Hanchuan Peng

  • 1Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.

BMC Bioinformatics
|March 19, 2013
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimental Validation and Bioinformatics Analysis Elucidate the Role of MTDH-Mediated PTEN Ubiquitination and Degradation in Podocyte Injury in Diabetic Kidney Disease.

Human mutation·2026
Same author

Gradient-based rigid motion correction in CBCT via Lie algebra-constrained registration.

Physics in medicine and biology·2026
Same author

BrainUMA: A Unified multi-atlas learning framework for brain disorders diagnosis.

Medical & biological engineering & computing·2026
Same author

C[Formula: see text]Net: A co-occurrence and consistency-aware framework for structured multi-label fundus diagnosis.

Medical & biological engineering & computing·2026
Same author

Size- and Time-Dependent Impacts of Polyvinyl Chloride Microplastics on Turbot (<i>Scophthalmus maximus</i> L.): Intestinal Tolerance, Hepatic Injury, and Intestinal Microbiota Dysbiosis.

Toxics·2026
Same author

From Ethnopharmacology to Drug Discovery: The Therapeutic Potential of Anisomeles indica.

Phytochemical analysis : PCA·2026
Same journal

OpenIMC: an open-source platform for analyzing single-cell and spatial proteomics by imaging mass cytometry.

BMC bioinformatics·2026
Same journal

NAP: an open source pipeline for cross-domain microbiome profiling using Nanopore sequencing-derived amplicon data.

BMC bioinformatics·2026
Same journal

SurvGME: an R package for survival analysis with graphical and measurement error models.

BMC bioinformatics·2026
Same journal

SimMapNet: a Bayesian framework for gene regulatory network inference using gene ontology similarities as external hint.

BMC bioinformatics·2026
Same journal

Dual channel drug-drug interactions extraction based on cross attention.

BMC bioinformatics·2026
Same journal

FeSseqdb: a curated sequence-level database and interpretable machine learning framework for identifying iron-sulfur proteins.

BMC bioinformatics·2026
See all related articles

DF-Tracing automatically reconstructs complex neuron 3D digital models from noisy microscopic images. This novel approach enhances neuron tracing accuracy for intricate cellular structures.

Area of Science:

  • Neuroscience
  • Computational Biology
  • Microscopy

Background:

  • Automatic 3D digital reconstruction of neurons from microscopic images is difficult.
  • Complex cell morphology and noisy images present significant challenges.

Purpose of the Study:

  • To develop an automated method for accurate 3D neuron reconstruction.
  • To overcome limitations in tracing complex neuronal structures in noisy images.

Main Methods:

  • DF-Tracing utilizes anisotropic filtering and automated thresholding to extract neurite signals.
  • A coupled distance-field (DF) algorithm with 'pressure' and 'thrust' force fields reconstructs neuron morphology.
  • The method employs a 'rolling ball' concept guided by distance transforms to trace the neuron skeleton.

More Related Videos

Automatic Identification of Dendritic Branches and their Orientation
06:08

Automatic Identification of Dendritic Branches and their Orientation

Published on: September 17, 2021

Dendritic Spine Quantification Using an Automatic Three-Dimensional Neuron Reconstruction Software
07:45

Dendritic Spine Quantification Using an Automatic Three-Dimensional Neuron Reconstruction Software

Published on: September 27, 2024

Related Experiment Videos

Last Updated: May 13, 2026

Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement
05:28

Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement

Published on: August 9, 2024

Automatic Identification of Dendritic Branches and their Orientation
06:08

Automatic Identification of Dendritic Branches and their Orientation

Published on: September 17, 2021

Dendritic Spine Quantification Using an Automatic Three-Dimensional Neuron Reconstruction Software
07:45

Dendritic Spine Quantification Using an Automatic Three-Dimensional Neuron Reconstruction Software

Published on: September 27, 2024

Main Results:

  • DF-Tracing successfully reconstructs intricate neuron structures from noisy 3D laser microscopy image stacks.
  • The algorithm automatically extracts neurite signals and reconstructs 3D morphology.

Conclusions:

  • DF-Tracing provides improved neuron reconstructions compared to previous methods.
  • The approach is effective for analyzing complex neuronal structures in biological samples like dragonfly thoracic ganglia.