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Nucleotide sequence of the gene encoding the matrix protein of mumps virus (Miyahara strain)
K Tanabayashi1, K Takeuchi, M Hishiyama
1Department of Measles Virus, National Institute of Health, Tokyo, Japan.
Abstract:
The nucleotide sequence of the gene encoding the matrix (M) protein of mumps virus (MuV), Miyahara strain, has been determined from several overlapping cDNA clones. The M protein mRNA is 1248 nucleotides in length, exclusive of the poly(A) tail, and codes for a protein of 375 amino acids (Mr41,556). Comparison of the deduced amino acid sequence of the M protein of the Miyahara strain with that of the SBL-1 strain revealed that the M proteins of both strains are highly conserved. A significantly lower rate of nucleotide differences conducive to amino acid differences in the M gene compared with other genes appeared to indicate the importance of the conserved primary structure of the M protein for its function.
Insights
The matrix (M) protein gene of mumps virus (MuV) was sequenced, revealing high conservation between Miyahara and SBL-1 strains. This suggests the M protein
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Mumps virus (MuV) is a significant human pathogen.
- The matrix (M) protein plays a crucial role in viral structure and assembly.
- Understanding MuV M protein's genetic characteristics is vital for virological research.
Purpose of the Study:
- To determine the nucleotide sequence of the M protein gene from the Miyahara strain of MuV.
- To analyze the deduced amino acid sequence and compare it with other MuV strains.
- To investigate the evolutionary conservation of the M protein and its functional implications.
Main Methods:
- Gene sequencing using overlapping cDNA clones.
- Bioinformatic analysis of nucleotide and amino acid sequences.
- Comparative sequence analysis between MuV strains.
Main Results:
- The M protein mRNA sequence for the Miyahara strain is 1248 nucleotides long.
- The M protein consists of 375 amino acids with a molecular weight of 41,556 Da.
- High amino acid sequence conservation was observed between the Miyahara and SBL-1 strains.
- The M gene exhibited a lower rate of nucleotide differences compared to other MuV genes, indicating functional importance.
Conclusions:
- The M protein of mumps virus is highly conserved across different strains.
- The conserved primary structure of the M protein is likely essential for its function.
- Further research into the M protein's role in MuV pathogenesis is warranted.