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Related Concept Videos

Volatilization01:10

Volatilization

Volatilization gravimetry is an analytical technique that measures the mass lost due to the volatilization of the substance. This technique is used to estimate the amount of volatile material in a sample. To perform this method, heat a known amount of the sample to a high temperature in a crucible or other suitable vessel. The volatile substance in the sample evaporates, and the vapor is completely expelled from the crucible either by heating the sample or bubbling a stream of inert gas through...

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Related Experiment Video

Updated: May 13, 2026

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
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Multiple headspace-solid-phase microextraction: an application to quantification of mushroom volatiles.

Rosaria Costa1, Laura Tedone, Selenia De Grazia

  • 1Dipartimento Farmaco-chimico, University of Messina, Messina, Italy.

Analytica Chimica Acta
|March 19, 2013
PubMed
Summary
This summary is machine-generated.

This study quantifies volatile compounds in champignon mushrooms (Agaricus bisporus) using multiple headspace-solid phase microextraction (MHS-SPME) and GC-MS/FID. Results reveal differences in volatile profiles influenced by sample preparation, impacting mushroom flavor analysis.

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Area of Science:

  • Food Chemistry
  • Analytical Chemistry
  • Mycology

Background:

  • Agaricus bisporus (champignon) is a widely consumed mushroom.
  • Understanding its volatile compounds is crucial for flavor and aroma analysis.
  • Accurate quantification of volatiles from solid food matrices presents analytical challenges.

Purpose of the Study:

  • To identify and quantify volatile compounds released by Agaricus bisporus.
  • To evaluate the effectiveness of multiple headspace-solid phase microextraction (MHS-SPME) for analyzing volatiles in mushroom samples.
  • To investigate the impact of sample preparation on volatile profiles.

Main Methods:

  • Multiple headspace-solid phase microextraction (MHS-SPME) for volatile extraction.
  • Gas chromatography/mass spectrometry (GC-MS) and gas chromatography/flame ionization detection (GC-FID) for separation and detection.
  • Analysis of fresh mushrooms and mushroom-containing food dressings.

Main Results:

  • Identified key volatile compounds including 1-octen-3-ol, 3-octanol, 3-octanone, 1-octen-3-one, and benzaldehyde.
  • Achieved method performance with low limits of detection (LoD) and quantification (LoQ), and high analyte recovery.
  • Observed quantitative differences in volatile profiles attributed to sample preparation techniques and cell damage.

Conclusions:

  • MHS-SPME is a suitable technique for the quantitative analysis of volatiles from solid mushroom matrices.
  • Sample preparation significantly influences the volatile profile of Agaricus bisporus.
  • Further research into mushroom biochemistry and MHS principles can refine volatile analysis.