Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 13, 2026

Evaluation of Motor Impairment in C. elegans Models of Amyotrophic Lateral Sclerosis
08:27

Evaluation of Motor Impairment in C. elegans Models of Amyotrophic Lateral Sclerosis

Published on: September 2, 2021

Molecular tools for studying the radial spoke.

Xiaoyan Zhu1, Yi Liu, Priyanka Sivadas

  • 1Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin, USA.

Methods in Enzymology
|March 19, 2013
PubMed
Summary

Researchers developed new methods to study the radial spoke in Chlamydomonas cilia and flagella, making complex axonemal biochemistry accessible for students and researchers. These strategies simplify the investigation of essential cellular structures.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Food Service Directors' Knowledge and Beliefs About Ultraprocessed Foods in California's San Joaquin Valley Schools.

Preventing chronic disease·2026
Same author

Cyanoacetylation of amines <i>via</i> a traceless cyanoacetyl radical: synthetic access to teriflunomide.

Chemical communications (Cambridge, England)·2026
Same author

Immobilized dead fungal biomass as a reusable biosorbent for reactive blue 19 and malachite green: kinetics, isotherms, and mechanistic insights.

World journal of microbiology & biotechnology·2026
Same author

Effect of patient position on digitally recorded interocclusal distance in prepared teeth: A computer-aided design and manufacturing-based evaluation.

Journal of Indian Prosthodontic Society·2026
Same author

Advances and Challenges in Vaccine Development for West Nile Virus (WNV) Infection.

Vaccines·2026
Same author

Eco-technological potential of salinity-driven functional specialization in Indian solar salterns revealed by integrated culturomics and whole-metagenome profiling.

BMC microbiology·2026

Area of Science:

  • Cell Biology
  • Biochemistry
  • Structural Biology

Background:

  • Cilia and flagella (9+2 axoneme) contain complex protein structures crucial for motility.
  • Biochemical analysis of these structures is challenging due to complexity and extensive molecular interactions.
  • Existing methods often require specialized training and expensive equipment, limiting broader research.

Purpose of the Study:

  • To describe strategies and methods for characterizing the radial spoke complex in Chlamydomonas axonemes.
  • To simplify the study of axonemal complexes for researchers with limited experience.
  • To provide adaptable methods for classroom settings and broader research applications.

Main Methods:

  • Utilized high-throughput databases and versatile research tools.

More Related Videos

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
08:31

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Published on: November 15, 2019

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
10:52

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

Published on: August 13, 2016

Related Experiment Videos

Last Updated: May 13, 2026

Evaluation of Motor Impairment in C. elegans Models of Amyotrophic Lateral Sclerosis
08:27

Evaluation of Motor Impairment in C. elegans Models of Amyotrophic Lateral Sclerosis

Published on: September 2, 2021

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
08:31

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Published on: November 15, 2019

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
10:52

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

Published on: August 13, 2016

  • Developed tailored strategies to overcome common experimental difficulties in axoneme biochemistry.
  • Focused on characterizing the radial spoke complex in Chlamydomonas.

    • Main Results:

    • Successfully circumvented common challenges in studying axonemal complexes.
    • Provided accessible methods for characterizing the radial spoke.
    • Demonstrated the adaptability of these methods for various axonemal components.

    Conclusions:

    • The developed strategies significantly lower the barrier to studying axonemal biochemistry.
    • These methods facilitate the investigation of novel axonemal complexes, including the radial spoke.
    • The approach is suitable for both student research and advanced laboratory settings.