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Related Concept Videos

Nuclear Protein Sorting01:34

Nuclear Protein Sorting

6.6K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
6.6K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.5K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

9.2K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
9.2K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

5.6K
No description available
5.6K
Nuclear Export01:42

Nuclear Export

5.2K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
5.2K
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

8.3K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
8.3K

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Related Experiment Video

Updated: Apr 1, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

12.3K

Uncovering nuclear pore complexity with innovation.

Rebecca L Adams1, Susan R Wente

  • 1Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.

Cell
|March 19, 2013
PubMed
Summary

High-resolution imaging and reductionist methods reveal complex nuclear pore transport mechanisms. Nucleoporin functions and specialized pathways drive these intricate cellular processes.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biophysics

Background:

  • The nuclear pore complex (NPC) regulates transport between the nucleus and cytoplasm.
  • Understanding NPC structure and function is crucial for cell biology.
  • Previous studies have focused on basic NPC structure and transport.

Purpose of the Study:

  • To elucidate the high-resolution structure of the NPC.
  • To understand the mechanistic complexities of nuclear transport.
  • To investigate the roles of nucleoporins in import and export pathways.

Main Methods:

  • Advanced imaging techniques (e.g., cryo-electron microscopy).
  • Reductionist approaches to dissect NPC components and functions.
  • Biochemical and biophysical analyses of nucleoporin interactions.

More Related Videos

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
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Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

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Single-Molecule Imaging of Nuclear Transport
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Single-Molecule Imaging of Nuclear Transport

Published on: June 10, 2010

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Related Experiment Videos

Last Updated: Apr 1, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

12.3K
Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
09:02

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

Published on: December 10, 2015

7.8K
Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 10, 2010

13.9K

Main Results:

  • High-resolution structural models of the NPC have been generated.
  • Unexpected mechanistic complexities in nuclear transport have been uncovered.
  • Specific nucleoporin functions and specialized import/export pathways have been identified.

Conclusions:

  • Modern imaging and reductionist strategies provide deep insights into NPC structure and transport.
  • Nucleoporin functions are key to the mechanistic intricacies of nuclear transport.
  • Specialized import and export pathways highlight the complexity of nucleocytoplasmic transport.